Banco de Germoplasma de la Universidad Rey Juan CarlosAcronym: BG URJC
El Banco de Germoplasma de la URJC se fundó en 2008 y actualmente consta de 392 accesiones y 191 especies. Está basado en la conservación de comunidades de plantas silvestres. El objetivo del banco es conservar ex situ una muestra representativa de toda la biodiversidad vegetal característica de un hábitat, haciendo énfasis en aquellos ecosistemas definidos como prioritarios por la Directiva de Hábitats. Además, se utiliza para almacenar material con fines investigadores y docentes. Está compuesto por dos subcolecciones típicas del centro peninsular: alta montaña (pastos psicroxerófilos) y semiárida (estepas gipsófilas), una subcolección representativa del género Lupinus y otra del género Cheirolophus procedente del archipiélago Canario. Todas las accesiones se conservan en tarros herméticos rellenos de silicagel guardados en congelador a -18ºC.
The Germplasm Bank of the URJC was created in 2008 and presently withholds 392 accessions and 191 species. It is based on the conservation of wild-plant communities. The aim of this bank is to conserve ex situ a representative sample of the plant biodiversity present in a habitat, putting an emphasis on those ecosystems identified as prioritary by the Habitats Directive. Moreover, it is used to store plant material for research and teaching purposes. It consists of two subcollections corresponding to habitats typical of the center of the Iberian peninsula: high mountain (psicroxerophylous pastures) and semi-arid (gypsophylic steppes), a representative subcollection of genus Lupinus and other of genus Cheirolophus from Canary Islands. All accesions are stored in the freezer at -18ºC in Kilner jars with silicagel.
High-mountain pastures (psicroxerophylous pastures) and semi-arid habitats (gypsophylic steppes) in the center of the Iberian Peninsula.
The URJC Germplasm Bank has a protocol which describes the seed processing methods for collection, processing in the laboratory, germination assays, dessication, recording accessions, labeling, scanning and freezing. Seed collectors are professors and researchers with great knowledge of the flora characteristic of each ecosystem. They are all members of research groups that have worked in this field for years and, therefore, have great experience in the phenology and identification of the species. Moreover, some accessions have herbarium specimens. 63% of the records are georreferenced with GPS and radio points. When this information is not available, the geographic coordinates of the site and the extension of the municipality are used as a surrogate of the measure.
Seed processing in the laboratory: The first treatment consisted of cleaning the seeds. Sieves with stainless steel meshes of different sizes (mesh holes between 2 mm-0.5 cm) were used to separate as much undesired material as possible (soil, stones, small leaves, stems, flowers, etc.) from the seed sample. The separated seeds were then introduced in tubes identified with the passport data (species name, date, site, altitude, collector) and stored in a cupboard at room temperature under ambient moisture conditions.
Germination assays: Germination assays were carried out with 100 seeds of each accession. Four replicates of twenty-five seeds were placed in Petri dishes on two pieces of filter paper. Distilled water was added until the surface was wet and the dishes were placed in a germination chamber (Selecta Hotcold GL, Barcelona, Spain). Accessions from the high mountain collection were incubated at 15ºC with a 16/8 hour light/dark photoperiod (Giménez-Benavides et al. 2005), while accessions from the semi-arid collection were incubated at 20ºC with the same photoperiod (Pérez-García and Durán 1989, Escudero et al. 1997, Herranz et al. 2002). Assays were monitored every 2-3 days for a minimum period of one month, and the assay was considered to have finished when no germination was observed for four subsequent censuses (8-day period). Germinated seeds were counted and removed in each census.
Dessication: Silica gel (SiO2) was used to desiccate the samples. As the desiccation rate increases with the amount of gel used, the base of the desiccator was filled to a 1:1 proportion with the seeds. The seeds which were separated by accessions in the Petri dishes were placed uncovered on the metal plate in the desiccator. They were then placed in a cool place to dry for at least two months.
Recording accessions: Accessions were added to the database of the URJC Germplasm Bank using Herbar Zoorbar Ligero (HZL) software. Each accession is assigned a reference number allocated consecutively. The information contained in the record of each accession is: dataset, institution, catalogue number, scientific name, family, genus, specific epithet, taxon rank, infraspecific epithet, scientific name authorship, collection code, number of mother plants, number of tubes (in which the accession is stored), number of seeds, seed weight, community name, country, country code, state province, municipality, locality, decimal latitude, decimal longitude, geodetic datum, coordinate uncertainty in meters, elevation, collector, number of jar (in which the accession is stored), number of drawer (in which the accession is stored), collection date, sample acquisition date, % germination and observations. All data are standardized by DarwinCore 1.2 and have been validated by Darwin Test. We generated metadata with DarwinCore Archive in order to publish data in GBIF IPT.
Labelling: After registering the accession in the database, seed accessions were labelled for proper identification.
Scanning: Approximately 50 seeds were uniformly distributed avoiding contact between them and scanned. A resolution of 300 ppp was used, except in the case of very small seeds when 600 ppp resolution was used.
Freezing: All of the collections in the URJC Germplasm Bank are base collections; i.e. they are stored for long periods of time and are only used in regeneration processes. Storage is carried out under optimal conditions to maximize seed viability. The procedure followed at the URJC Germplasm Bank was to store seeds in glass tubes in Kilner jars with silica gel. These jars were sealed hermetically and placed in a freezer at -18ºC.
Type of content
Includes: point occurrence data.
Universidad Rey Juan Carlos (2013 -). URJC GB dataset: Community-based seed bank of Mediterranean high-mountain and semi-arid plant species at Universidad Rey Juan Carlos (Spain), 235 data records, Online, http://www.gbif.es:8080/ipt/resource.do?r=germoplasma-urjc, Version 3.0 (last updated on 02/08/2018)
This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.
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Metadata last updated on 2020-04-08 09:19:32.0